Microbiology is making significant contributions towards scientific knowledge and solving human problems nowadays. In order to be an accomplished biotechnologist, a thorough knowledge of Microbiology is essential. Microorganisms were probably the first living forms to appear on the earth. The present manual comes from the teachers and Scholars who have good experience in teaching and research in microbiology & Biological Sciences. This Manual is very useful to Students who work in the discipline in Microbiology. The Book contains basic techniques regarding to Microbiology to both Graduates & Post Graduates levels. The experiments have been given in chronological orders and most of these can be performed with materials available in most biological sciences laboratories. For some of the experiments even alternate procedures have been suggested. The experiments covered in this manual are easy to perform, and have been used over the years in our laboratory for imparting practical training regularly to undergraduates and postgraduate students.Experiments were written in a step wise manner for easy understanding. Suggestions from readers and users are welcome.