Recent developments in molecular biology and biochemistry have made it possible to determine the three-dimensional structure of membrane proteins and so come to a full understanding of their function. In Membrane Protein Protocols: Expression, Purification, and Characterization, researchers at major universities and research centers around the world describe in detail the key techniques that have proven successful in the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
Timely and highly practical, Membrane Protein Protocols: Expression, Purification, and Characterization offers investigators proven methods to determine the three-dimensional structure of membrane receptors and transport proteins, as well as proven practical help in the discovery of new clinical targets for the design of novel pharmaceutical agents useful in treating cancer, bacterial and viral infection, and many metabolic diseases.