Bioimage analysis is a crucial field at the intersection of biology, computer science, and mathematics. It involves extracting quantitative information from images generated by various biological imaging techniques such as microscopy, MRI, and CT scans. This multidisciplinary approach enables researchers to understand complex biological phenomena at the cellular and molecular levels. In bioimage analysis, sophisticated algorithms are employed to process and analyze large volumes of image data, allowing for the detection and quantification of biological structures, cellular processes, and interactions within living organisms. These analyses facilitate the study of diverse biological processes, including cell division, protein localization, tissue development, and disease progression. Moreover, bioimage analysis plays a vital role in advancing medical diagnostics and therapeutics. By providing insights into the intricate workings of biological systems, it aids in the discovery of novel biomarkers, drug targets, and therapeutic interventions. The topics included in this book on bioimage analysis are of the utmost significance and bound to provide incredible insights to readers. Most of the topics introduced herein cover new techniques and the applications of bioimage analysis. This book, with its detailed coverage, will prove immensely beneficial to professionals and students involved in this area at various levels.