This outstanding and comprehensive collection of user-friendly protocols is essential for neuroscientists who wish to localize, identify, purify, and/or quantify neurotransmitters. The easily reproducible methods presented cover a broad spectrum of approaches involving cell and organ culture, histochemistry, immunochemistry, in situ hybridization, cell fractionation, liquid chromatography, mass spectrometry, in vivo microdialysis, and voltammetry. These approaches are optimized for use by neuroscientists, are often complementary, and can be employed in tandem with powerful effect.