In the course of biosurfactant production, it would be economical to carry out optimization procedures so as to determine the best combination of substrate concentration and culture conditions that would lead to maximum biosurfactant yield. This will ensure that wastage of resources such as nutrient and production time is avoided.This book provides a guide for carrying out the various steps in the optimization of biosurfactant production. Directions for performing the calculations involved and plotting the response surfaces using the Microsoft excel platform are provided in this book. Although there are softwares available for generating response plots from mere input of experimental data, having a feel of the calculations involved will provide the experimenter with the knowledge of the procedures involved in the optimization process.Topics covered in this book include (1) Culturing microorganisms for biosurfactant production, (2) Screening for biosurfactant production, (3) Procedures in optimization of biosurfactant production, and (4) Extraction of biosurfactants. These will be valuable to researchers and industrialist venturing into biosurfactant production.