Poly hydroxyl butyrate (PHB) can be used as an effective thermoplastic, and has many characteristics similar to those of standard commercial plastics like polypropylene. PHB- based plastic substitutes are less flexible than traditional plastics; they are completely biodegradable and leave behind no residue. The present study deals with the isolation, screening, production and extraction of Polyhydroxy butyrate from bacillus species. And characterization of biopolymer (PHB) producing bacteria from different soil sample in and around the Bangalore. Out of a total of 3 isolated strains one strain was selected for PHB production in different conditions like incubation temperature and time. The bacteria were screened by morphological and biochemical characteristics as Bacillus species. The bacteria were further identified by Sudan black- B staining and molecular characterization. Regarding incubation time, temperature, pH, and inoculum size optimum PHB production conditions were 48hour 30°C and 7.0, size1, respectively. The present study provides the useful industrial production of PHB, a fast-emerging alternative of non-biodegradable plastics.